evaluation of immune status to measles in vaccinated population in tehran, using enzyme-linked immunosorbent assay and the hemagglutination inhibition techniques
نویسندگان
چکیده
abstract: measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including iran. understanding measles outbreaks that occur after the initiation of measles elimination efforts will be critical in refining the strategies for measles elimination. the present study was performed to evaluate the immune status against measles after the mass campaign vaccination in 2003.in this study, approximately 172 sera were analysed by elisa and hi tests. the results indicated, 162 were positive (94.2%) and 10 were negative (5.8%) by hi test and 165 were positive (95.5%) and 7 were negative (4.1%) by the elisa test.
منابع مشابه
Evaluation of immune status to measles in vaccinated population in Tehran, using enzyme-linked immunosorbent assay and the hemagglutination inhibition techniques
Abstract: Measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. Although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including Iran. Understanding measles outbreaks that occur after the initiation of measles eliminati...
متن کاملEvaluation of Immune Status to Measles in Vaccinated Population in Tehran, by Enzyme-linked Immunosorbent Assay and the Hemagglutination Inhibition Techniques (1386-1387)
Background and Aims: Measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. Although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including Iran. Methods: The present study was performed to evaluate the immune status aga...
متن کاملevaluation of immune status to measles in vaccinated population in tehran, by enzyme-linked immunosorbent assay and the hemagglutination inhibition techniques (1386-1387)
background and aims: measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including iran. methods: the present study was performed to evaluate the immune status agai...
متن کاملENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
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Hemagglutination inhibition, enzyme-linked immunosorbent assay (ELISA), and passive latex agglutination were used to test 495 human serum samples for determination of rubella immunity. Overall agreements for immune status were as follows: hemagglutination inhibition versus ELISA, 94.7% (469 of 495); hemagglutination inhibition versus passive latex agglutination (1:10 dilution), 99.2% (491 of 49...
متن کاملComparison of measles antihemolysin test, enzyme-linked immunosorbent assay, and hemagglutination inhibition test with neutralization test for determination of immune status.
Sera were collected from 238 high-school students in Prince Edward Island for the determination of immune status before an anticipated measles outbreak. In addition, history of vaccination status and measles infection was obtained. In the subsequent outbreak, 28 students did contract measles. Specificity for hemagglutination inhibition (HI), antihemolysin (AH), and enzyme-linked immunosorbent a...
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عنوان ژورنال:
iranian journal of virologyجلد ۱، شماره ۴، صفحات ۲۷-۳۰
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